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Binding and cross-linking of tmRNA to ribosomal protein S1, on and off the Escherichia coli ribosome

UV irradiation of an in vitro translation mixture induced cross-linking of 4-thioU-substituted tmRNA to Escherichia coli ribosomes by forming covalent complexes with ribosomal protein S1 and 16S rRNA. In the absence of S1, tmRNA was unable to bind and label ribosomal components. Mobility assays on n...

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Detalles Bibliográficos
Main Authors: Wower, Iwona K., Zwieb, Christian W., Guven, Sadel A., Wower, Jacek
Formato: Artigo
Idioma:Inglês
Publicado: Oxford University Press 2000
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC305868/
https://ncbi.nlm.nih.gov/pubmed/11101533
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/emboj/19.23.6612
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