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Robust RT-qPCR Data Normalization: Validation and Selection of Internal Reference Genes during Post-Experimental Data Analysis

Reverse transcription and real-time PCR (RT-qPCR) has been widely used for rapid quantification of relative gene expression. To offset technical confounding variations, stably-expressed internal reference genes are measured simultaneously along with target genes for data normalization. Statistic met...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Ling, Daijun, Salvaterra, Paul M.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Public Library of Science 2011
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3058000/
https://ncbi.nlm.nih.gov/pubmed/21423626
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0017762
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