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Parallel Detection of Intrinsic Fluorescence from Peptides and Proteins for Quantification During Mass Spectrometric Analysis

Direct mass spectrometric quantification of peptides and proteins is compromised by the wide variabilities in ionization efficiency which are hallmarks of both the MALDI and ESI ionization techniques. We describe here the implementation of a fluorescence detection system for measurement of the UV-ex...

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Detalles Bibliográficos
Main Authors: Russell, Jason D., Hilger, Ryan T., Ladror, Daniel T., Tervo, Mark A., Scalf, Mark, Shortreed, Michael R., Coon, Joshua J., Smith, Lloyd M.
Formato: Artigo
Idioma:Inglês
Publicado: 2011
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3056920/
https://ncbi.nlm.nih.gov/pubmed/21314137
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac103023q
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