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Using differential solubilization and 2-D gel electrophoresis to visualize increased numbers of proteins in the human cortex and caudate nucleus and putamen

The aim of this study was to determine if differential solubilization of human CNS proteins would increase the total number of proteins that could be visualized using 2-D gel electrophoresis. Hence, proteins were solubilized into Tris, CHAPS and SB3–10 before separation across a pH 4–7 IEF gradient...

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Detalhes bibliográficos
Main Authors: Dean, Brian, Pavey, Geoffrey, Smith, A. Ian
Formato: Artigo
Idioma:Inglês
Publicado em: 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3056166/
https://ncbi.nlm.nih.gov/pubmed/21136922
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/prca.200800037
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