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Using Fluorophore-labeled Oligonucleotides to Measure Affinities of Protein-DNA Interactions
Changes in fluorescence emission intensity and anisotropy can reflect changes in the environment and molecular motion of a fluorophore. Researchers can capitalize on these characteristics to assess the affinity and specificity of DNA-binding proteins using fluorophore-labeled oligonucleotides. While...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2008
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3051356/ https://ncbi.nlm.nih.gov/pubmed/19152864 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/S0076-6879(08)03412-5 |
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