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cDNA cloning of a serotonin 5-HT1C receptor by electrophysiological assays of mRNA-injected Xenopus oocytes.

We describe a strategy for the cloning of neurotransmitter-receptor and ion-channel cDNAs that is based on electrophysiological assays of mRNA-injected Xenopus oocytes. This procedure circumvents the purification of these membrane proteins, which is hindered by their low abundance and their hydropho...

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Detalhes bibliográficos
Main Authors: Lübbert, H, Hoffman, B J, Snutch, T P, van Dyke, T, Levine, A J, Hartig, P R, Lester, H A, Davidson, N
Formato: Artigo
Idioma:Inglês
Publicado em: 1987
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC305079/
https://ncbi.nlm.nih.gov/pubmed/3473504
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