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Strand-specific real-time RT-PCR for distinguishing influenza vRNA, cRNA, and mRNA
Real-time RT-PCR is used to quantify individual influenza viral RNAs. However, conventional real-time RT-PCR, using strand-specific primers, has been shown to produce not only the anticipated strand-specific products, but also substantial amounts of non-strand-specific products, indicating lack of s...
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| Principais autores: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3049850/ https://ncbi.nlm.nih.gov/pubmed/21185869 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jviromet.2010.12.014 |
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