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Replication of mini-P1 plasmid DNA in vitro requires two initiation proteins, encoded by the repA gene of phage P1 and the dnaA gene of Escherichia coli.

We have developed an in vitro DNA-replication system that replicates exogenously added mini-P1 plasmid DNA. The system consists of purified P1 RepA protein and a partially purified mixture of Escherichia coli replication proteins. It is essentially the same as that described for the replication of o...

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Detalles Bibliográficos
Autores principales: Wickner, S H, Chattoraj, D K
Formato: Artigo
Lenguaje:Inglês
Publicado: 1987
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC304936/
https://ncbi.nlm.nih.gov/pubmed/3035546
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