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Screening an expression library with a ligand probe: isolation and sequence of a cDNA corresponding to a brain calmodulin-binding protein.

The use of cloning vectors that express inserted cDNA as fusion protein has led to the isolation of genes encoding a variety of eukaryotic proteins. In these instances antisera or monoclonal antibodies were used as probes to screen expression libraries. Since fusion proteins sometimes display biolog...

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Bibliografiske detaljer
Main Authors: Sikela, J M, Hahn, W E
Format: Artigo
Sprog:Inglês
Udgivet: 1987
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC304797/
https://ncbi.nlm.nih.gov/pubmed/3033675
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