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Site-specific (19)F NMR chemical shift and side chain relaxation analysis of a membrane protein labeled with an unnatural amino acid

Site-specific (19)F chemical shift and side chain relaxation analysis can be applied on large size proteins. Here, one-dimensional (19)F spectra and T(1), T(2) relaxation data were acquired on a SH3 domain in aqueous buffer containing 60% glycerol, and a nine-transmembrane helices membrane protein d...

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Detalhes bibliográficos
Main Authors: Shi, Pan, Wang, Hu, Xi, Zhaoyong, Shi, Chaowei, Xiong, Ying, Tian, Changlin
Formato: Artigo
Idioma:Inglês
Publicado em: Wiley Subscription Services, Inc., A Wiley Company 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3047079/
https://ncbi.nlm.nih.gov/pubmed/21080424
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.545
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