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Restrained expression, a method to overproduce toxic membrane proteins by exploiting operator–repressor interactions
A major rate-limiting step in determining structures of membrane proteins is heterologous protein production. Toxicity often associated with rapid overexpression results in reduced biomass along with low yields of target protein. Mitigation of toxic effects was achieved using a method we call “restr...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Wiley Subscription Services, Inc., A Wiley Company
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3047061/ https://ncbi.nlm.nih.gov/pubmed/21031485 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.535 |
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