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Specific binding of [alpha-32P]GTP to cytosolic and membrane-bound proteins of human platelets correlates with the activation of phospholipase C.

We have assessed the binding of [alpha-32P]GTP to platelet proteins from cytosolic and membrane fractions. Proteins were separated by NaDodSO4/PAGE and electrophoretically transferred to nitrocellulose. Incubation of the nitrocellulose blots with [alpha-32P]GTP indicated the presence of specific and...

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Autors principals: Lapetina, E G, Reep, B R
Format: Artigo
Idioma:Inglês
Publicat: 1987
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC304629/
https://ncbi.nlm.nih.gov/pubmed/3470789
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