Selective ploidy ablation, a high-throughput plasmid transfer protocol, identifies new genes affecting topoisomerase I–induced DNA damage

We have streamlined the process of transferring plasmids into any yeast strain library by developing a novel mating-based, high-throughput method called selective ploidy ablation (SPA). SPA uses a universal plasmid donor strain that contains conditional centromeres on every chromosome. The plasmid-b...

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Bibliographische Detailangaben
Hauptverfasser: Reid, Robert J.D., González-Barrera, Sergio, Sunjevaric, Ivana, Alvaro, David, Ciccone, Samantha, Wagner, Marisa, Rothstein, Rodney
Format: Artigo
Sprache:Inglês
Veröffentlicht: Cold Spring Harbor Laboratory Press 2011
Schlagworte:
Method
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3044861/
https://ncbi.nlm.nih.gov/pubmed/21173034
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.109033.110
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