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Isolation of high-affinity memory B cells: phycoerythrin as a probe for antigen-binding cells.
In multiparameter fluorescence-activated cell sorter studies presented here, we use the highly fluorescent protein phycoerythrin (PE) as an immunizing antigen and as a fluorescent probe to reveal and isolate antigen-binding memory B cells. We demonstrate directly that memory B cells in spleens from...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1987
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC304433/ https://ncbi.nlm.nih.gov/pubmed/3493491 |
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