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Isolation of temperature-sensitive tyrosine kinase mutants of v-abl oncogene by screening with antibodies for phosphotyrosine.

Temperature-sensitive protein-tyrosine kinase (EC 2.7.1.112) mutants of the oncogene v-abl have been obtained by a direct screening of kinase mutants in bacteria. The v-abl oncogene was expressed in Escherichia coli as a trpE/v-abl fusion protein from the trp promoter. The expression plasmid was mut...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Kipreos, E T, Lee, G J, Wang, J Y
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1987
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC304425/
https://ncbi.nlm.nih.gov/pubmed/2434953
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