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Proton NMR measurements of bacteriophage T4 lysozyme aided by 15N isotopic labeling: structural and dynamic studies of larger proteins.

A strategy for resolution and assignment of single proton resonances in proteins of molecular mass up to at least 40 kDa is presented. This approach is based on 15N (or 13C) labeling of selected residues in a protein. The resonances from protons directly bonded to labeled atoms are detected in a two...

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Détails bibliographiques
Auteurs principaux: McIntosh, L P, Griffey, R H, Muchmore, D C, Nielson, C P, Redfield, A G, Dahlquist, F W
Format: Artigo
Langue:Inglês
Publié: 1987
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC304403/
https://ncbi.nlm.nih.gov/pubmed/3029773
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