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Quantitative Experimental Assessment of Macromolecular Crowding Effects at Membrane Surfaces
We examined how crowding of the surfaces of lipid vesicles with either grafted polyethyleneglycol (PEG) chains or bilayer-anchored protein molecules affects the binding of soluble proteins to the vesicle surface. Escherichia coli dihydrofolate reductase (DHFR, 18 kDa) or a larger fusion protein, Nus...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
The Biophysical Society
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3042566/ https://ncbi.nlm.nih.gov/pubmed/20923646 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2010.07.047 |
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