Solubilization of a membrane-bound diol dehydratase with retention of EPR g = 2.02 signal by using 2-(N-cyclohexylamino)ethanesulfonic acid buffer.

A procedure for solubilization of the oxygen-labile, membrane-bound diol dehydratase from Clostridium glycolicum with retention of enzymatic activity is described. The procedure involves sonication of crude membrane preparations anaerobically in 0.1 M 2-(N-cyclohexylamino)ethane-sulfonic acid (CHES)...

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Bibliografiske detaljer

Udgivet i:	Proc Natl Acad Sci U S A
Principais autores:	Hartmanis, M G, Stadtman, T C
Format:	Artigo
Sprog:	Inglês
Udgivet:	National Academy of Sciences 1987
Fag:	Research Article
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC304144/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3025865/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.84.1.76
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