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A replaceable microreactor for on-line protein digestion in a two-dimensional capillary electrophoresis system with tandem mass spectrometry detection

We describe a two-dimensional capillary electrophoresis system that incorporates a replaceable enzymatic microreactor for on-line protein digestion. In this system, trypsin is immobilized on magnetic beads. At the start of each experiment, old beads are flushed to waste and replaced with a fresh plu...

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Main Authors: Li, Yihan, Wojcik, Roza, Dovichi, Norman J.
Format: Artigo
Jezik:Inglês
Izdano: 2010
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3038187/
https://ncbi.nlm.nih.gov/pubmed/21030030
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.chroma.2010.10.013
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