Crystallization and preliminary crystallographic analysis of β-mannanase from Bacillus licheniformis

The mannan endo-1,4-β-mannosidase (ManB) from Bacillus licheniformis strain DSM13 was overexpressed in Escherichia coli. Purification of the thermostable and alkali-stable recombinant mannanase yielded approximately 50 mg enzyme per litre of culture. Crystals were grown by hanging-drop vapour diffus...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Songsiriritthigul, Chomphunuch, Lapboonrueng, Sasithorn, Roytrakul, Sittiruk, Haltrich, Dietmar, Yamabhai, Montarop
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: International Union of Crystallography 2011
Gaiak:
Crystallization Communications
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC3034611/
https://ncbi.nlm.nih.gov/pubmed/21301089
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309110049067
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