Rapid Genetic Engineering of Human Cytomegalovirus by Using a Lambda Phage Linear Recombination System: Demonstration that pp28 (UL99) Is Essential for Production of Infectious Virus

A highly efficient lambda phage recombination system previously utilized for studies of bacterial artificial chromosome (BAC)-maintained mouse chromosomal DNA was adapted for the study of the role of human cytomegalovirus (HCMV)-encoded pp28 (UL99) in virus replication. Incorporating a two-step muta...

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Detalhes bibliográficos
Main Authors: Britt, William J., Jarvis, Michael, Seo, Jun-Young, Drummond, Derek, Nelson, Jay
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2004
Assuntos:
Structure and Assembly
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC303398/
https://ncbi.nlm.nih.gov/pubmed/14671136
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JVI.78.1.539-543.2004
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