Development of an in situ assay for simultaneous detection of the genomic and replicative form of PCV2 using padlock probes and rolling circle amplification

BACKGROUND: In this study we utilized padlock probes and rolling circle amplification as a mean to detect and study the replication of porcine circovirus type 2 (PCV2) in cultured cells and in infected tissue. Porcine circovirus type 2 is a single-stranded circular DNA virus associated with several...

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Bibliographic Details
Main Authors: Henriksson, Sara, Blomström, Anne-Lie, Fuxler, Lisbeth, Fossum, Caroline, Berg, Mikael, Nilsson, Mats
Format: Artigo
Language:Inglês
Published: BioMed Central 2011
Subjects:
Methodology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3033839/
https://ncbi.nlm.nih.gov/pubmed/21261961
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1743-422X-8-37
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