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Cytochrome P450 from Photobacterium profundum SS9, a piezophilic bacterium, exhibits a tightened control of water access to the active site

We report cloning, expression in E. coli, and purification of cytochrome P450 from a deep-sea bacterium Photobacterium profundum strain SS9 (P450-SS9). The enzyme, which is predominately high-spin (86%) in the absence of any added ligand, binds fatty acids and their derivatives and exhibits the high...

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Sonraí Bibleagrafaíochta
Main Authors: Sineva, Elena V., Davydov, Dmitri R.
Formáid: Artigo
Teanga:Inglês
Foilsithe: 2010
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3027307/
https://ncbi.nlm.nih.gov/pubmed/21082780
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi101466y
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