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Cytochrome P450 from Photobacterium profundum SS9, a piezophilic bacterium, exhibits a tightened control of water access to the active site
We report cloning, expression in E. coli, and purification of cytochrome P450 from a deep-sea bacterium Photobacterium profundum strain SS9 (P450-SS9). The enzyme, which is predominately high-spin (86%) in the absence of any added ligand, binds fatty acids and their derivatives and exhibits the high...
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| Main Authors: | , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2010
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3027307/ https://ncbi.nlm.nih.gov/pubmed/21082780 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi101466y |
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