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A place for everything: Chromosomal integration of large constructs
We have developed an easy, reliable two-step method for the insertion of large DNA fragments into any desired location in the E. coli chromosome. The method is based on the recombineering of a small (∼1.3 kbp) “Landing Pad” into the chromosome at the insertion site, to which the large construct is s...
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| Auteurs principaux: | , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Landes Bioscience
2010
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3026472/ https://ncbi.nlm.nih.gov/pubmed/21327065 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4161/bbug.1.4.12386 |
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