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The use of caspase inhibitors in pulsed-field gel electrophoresis may improve the estimation of radiation-induced DNA repair and apoptosis

BACKGROUND: Radiation-induced DNA double-strand break (DSB) repair can be tested by using pulsed-field gel electrophoresis (PFGE) in agarose-encapsulated cells. However, previous studies have reported that this assay is impaired by the spontaneous DNA breakage in this medium. We investigated the mec...

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Detalhes bibliográficos
Main Authors: Balart, Josep, Pueyo, Gemma, de Llobet, Lara I, Baro, Marta, Sole, Xavi, Marin, Susanna, Casanovas, Oriol, Mesia, Ricard, Capella, Gabriel
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2011
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3025872/
https://ncbi.nlm.nih.gov/pubmed/21235815
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1748-717X-6-6
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