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Genome-Scale Identification of Resistance Functions in Pseudomonas aeruginosa Using Tn-seq
We describe a deep-sequencing procedure for tracking large numbers of transposon mutants of Pseudomonas aeruginosa. The procedure employs a new Tn-seq methodology based on the generation and amplification of single-strand circles carrying transposon junction sequences (the Tn-seq circle method), a m...
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| Main Authors: | , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Society of Microbiology
2011
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3023915/ https://ncbi.nlm.nih.gov/pubmed/21253457 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/mBio.00315-10 |
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