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A PCR-based cloning strategy applicable to functional microRNA studies
MicroRNAs (miRNAs) are key regulatory RNAs that act in concert to coordinately repress messenger RNA (mRNA) translation through imperfect recognition of multiple specific binding sites (BS) located in their 3′ untranslated region (UTR). Here, we present a polymerase-chain reaction (PCR)-based clonin...
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| Autores principales: | , , , |
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| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
2008
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3023812/ https://ncbi.nlm.nih.gov/pubmed/18606151 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2008.06.026 |
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