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Production and purification of VP2 protein of porcine parvovirus expressed in an insect-baculovirus cell system
The porcine parvovirus (PPV) VP2 protein was expressed in an insect-baculovirus cell system and was purified using Ni-NTA affinity column chromatography. The recombinant 6-His-tagged VP2 protein with molecular mass (Mr) of about 64 kDa was detected by anti-his antibody and anti-PPV serum. Electron m...
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Main Authors: | , , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
BioMed Central
2010
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3022681/ https://ncbi.nlm.nih.gov/pubmed/21143963 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1743-422X-7-366 |
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