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Relative and Absolute Quantitative Real-Time PCR-Based Quantifications of hcnC and phlD Gene Transcripts in Natural Soil Spiked with Pseudomonas sp. Strain LBUM300

Transcriptional analysis of microbial gene expression using relative quantitative real-time PCR (qRT-PCR) has been hampered by various technical problems. One such problem is the unavailability of an exogenous standard robust enough for use in a complex matrix like soil. To circumvent this technical...

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Bibliografische gegevens
Hoofdauteurs: DeCoste, Nadine J., Gadkar, Vijay J., Filion, Martin
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: American Society for Microbiology (ASM) 2011
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3019740/
https://ncbi.nlm.nih.gov/pubmed/21075889
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01387-10
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