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An efficient system to generate monoclonal antibodies against membrane-associated proteins by immunisation with antigen-expressing mammalian cells
BACKGROUND: The generation of monoclonal antibodies specific for protein antigens usually depends on purified recombinant protein for both immunisation and hybridoma screening. Purification of recombinant protein in sufficient yield and purity is a tedious undertaking and can be demanding especially...
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Main Authors: | , , , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
BioMed Central
2010
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Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3019159/ https://ncbi.nlm.nih.gov/pubmed/21159168 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-10-87 |
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