Spatiotemporal dynamics of guanosine 3′,5′-cyclic monophosphate revealed by a genetically encoded, fluorescent indicator

To investigate the dynamics of guanosine 3′,5′-cyclic monophosphate (cGMP) in single living cells, we constructed genetically encoded, fluorescent cGMP indicators by bracketing cGMP-dependent protein kinase (cGPK), minus residues 1–77, between cyan and yellow mutants of green fluorescent protein. cG...

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Bibliographic Details
Main Authors: Honda, Akira, Adams, Stephen R., Sawyer, Carolyn L., Lev-Ram, Varda, Tsien, Roger Y., Dostmann, Wolfgang R. G.
Format: Artigo
Language:Inglês
Published: The National Academy of Sciences 2001
Subjects:
Biological Sciences
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC30156/
https://ncbi.nlm.nih.gov/pubmed/11226257
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.051631298
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