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Kinetics of protein import into isolated Xenopus oocyte nuclei

An in vitro assay for nucleocytoplasmic transport was established in which signal-dependent protein import is reproduced faithfully by isolated purified nuclei. The assay permits the precise quantification of import kinetics and the discrimination between translocation through the nuclear envelope a...

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Main Authors: Radtke, Thomas, Schmalz, Dirk, Coutavas, Elias, Soliman, Tarik M., Peters, Reiner
Format: Artigo
Jezik:Inglês
Izdano: The National Academy of Sciences 2001
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC30151/
https://ncbi.nlm.nih.gov/pubmed/11226252
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.051616598
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