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Perfluorooctanoic Acid for Shotgun Proteomics

Here, we describe the novel use of a volatile surfactant, perfluorooctanoic acid (PFOA), for shotgun proteomics. PFOA was found to solubilize membrane proteins as effectively as sodium dodecyl sulfate (SDS). PFOA concentrations up to 0.5% (w/v) did not significantly inhibit trypsin activity. The uni...

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Hlavní autoři: Kadiyala, Chandra Sekhar Rao, Tomechko, Sara E., Miyagi, Masaru
Médium: Artigo
Jazyk:Inglês
Vydáno: Public Library of Science 2010
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3012695/
https://ncbi.nlm.nih.gov/pubmed/21209883
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0015332
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