Silent Mischief: Bacteriophage Mu Insertions Contaminate Products of Escherichia coli Random Mutagenesis Performed Using Suicidal Transposon Delivery Plasmids Mobilized by Broad-Host-Range RP4 Conjugative Machinery

Random transposon mutagenesis is the strategy of choice for associating a phenotype with its unknown genetic determinants. It is generally performed by mobilization of a conditionally replicating vector delivering transposons to recipient cells using broad-host-range RP4 conjugative machinery carrie...

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Detalhes bibliográficos
Main Authors: Ferrières, Lionel, Hémery, Gaëlle, Nham, Toan, Guérout, Anne-Marie, Mazel, Didier, Beloin, Christophe, Ghigo, Jean-Marc
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2010
Assuntos:
Genetics and Molecular Biology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3008518/
https://ncbi.nlm.nih.gov/pubmed/20935093
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.00621-10
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