A SUMO-GROUCHO Q DOMAIN FUSION PROTEIN: CHARACTERIZATION AND IN VIVO ULP1-MEDIATED CLEAVAGE

We describe here a system for the expression and purification of small ubiquitin-related modifier (SUMO) fusion proteins, which often exhibit dramatically increased solubility and stability during expression in bacteria relative to unfused proteins. The vector described here allows expression of a H...

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Detaylı Bibliyografya
Asıl Yazarlar: Kuo, Dennis, Nie, Minghua, De Hoff, Peter, Chambers, Michael, Phillips, Martin, Hirsch, Ann M., Courey, Albert J.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 2010
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Article
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC3005967/
https://ncbi.nlm.nih.gov/pubmed/20732424
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2010.08.008
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