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Experimental optimization of protein refolding with a genetic algorithm

Refolding of proteins from solubilized inclusion bodies still represents a major challenge for many recombinantly expressed proteins and often constitutes a major bottleneck. As in vitro refolding is a complex reaction with a variety of critical parameters, suitable refolding conditions are typicall...

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Detalhes bibliográficos
Main Authors: Anselment, Bernd, Baerend, Danae, Mey, Elisabeth, Buchner, Johannes, Weuster-Botz, Dirk, Haslbeck, Martin
Formato: Artigo
Idioma:Inglês
Publicado em: Wiley Subscription Services, Inc., A Wiley Company 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3005780/
https://ncbi.nlm.nih.gov/pubmed/20799347
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.488
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