Activated-Ion ETD (AI-ETD) Improves the Ability of ETD to Identify Peptides in a Complex Mixture

Using a modified ETD-enabled QLT mass spectrometer, we demonstrate the utility of IR activation concomitant with ETD ion-ion reactions (activated-ion ETD, AI-ETD). Analyzing 12 SCX fractions of a LysC digest of human cells (HS) using ETD, CAD, and AI-ETD, we find that AI-ETD generates 13,405 peptide...

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Main Authors: Ledvina, Aaron R., Beauchene, Nicole A., McAlister, Graeme C., Syka, John E. P., Schwartz, Jae C., Griep-Raming, Jens, Westphall, Michael S., Coon, Joshua J.
Format: Artigo
Sprog:Inglês
Udgivet: 2010
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3005528/
https://ncbi.nlm.nih.gov/pubmed/21062032
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac1020358
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