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Quantitative PCR used to Assess HIV-1 Integration and 2-LTR Circle Formation in Human Macrophages, Peripheral Blood Lymphocytes and a CD4+ Cell Line

BACKGROUND: Integration is an intermediate step in the HIV life cycle and is defined as the insertion of HIV-1 proviral DNA into the host chromosome. If integration does not occur when HIV-1 cDNA enters the nucleus, it circularizes upon itself and forms a 2-LTR circle. Monitoring the level of integr...

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Detalhes bibliográficos
Main Authors: Friedrich, Brian, Li, Guangyu, Dziuba, Natallia, Ferguson, Monique R
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3003270/
https://ncbi.nlm.nih.gov/pubmed/21129188
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1743-422X-7-354
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