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Mapping of the nuclear matrix-bound chromatin hubs by a new M3C experimental procedure
We have developed an experimental procedure to analyze the spatial proximity of nuclear matrix-bound DNA fragments. This protocol, referred to as Matrix 3C (M3C), includes a high salt extraction of nuclei, the removal of distal parts of unfolded DNA loops using restriction enzyme treatment, ligation...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3001081/ https://ncbi.nlm.nih.gov/pubmed/20705651 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkq712 |
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