Crystallization and preliminary X-ray analysis of 5-­keto-d-gluconate reductase from Gluconobacter suboxydans IFO12528 complexed with 5-keto-d-gluconate and NADPH

NADPH-dependent 5-keto-d-gluconate reductase from Gluconobacter suboxy­dans IFO12528 (5KGR) catalyzes oxidoreduction between 5-keto-d-gluconate and d-gluconate with high specificity. 5KGR was expressed in Escherichia coli, purified and crystallized with 5-keto-d-gluconate and NADPH using the sitting...

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Detalhes bibliográficos
Main Authors: Kubota, Keiko, Miyazono, Ken-ichi, Nagata, Koji, Toyama, Hirohide, Matsushita, Kazunobu, Tanokura, Masaru
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2010
Assuntos:
Crystallization Communications
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2998383/
https://ncbi.nlm.nih.gov/pubmed/21139224
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309110043617
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