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Expression, purification, crystallization and initial X-ray diffraction analysis of thiol peroxidase from Yersinia pseudotuberculosis

Thiol peroxidase is an atypical 2-Cys peroxiredoxin that reduces alkyl hydroperoxides. Wild-type and C61S mutant protein have been recombinantly expressed in Escherichia coli and purified using nickel-affinity chromatography. Initial crystallization trials yielded three crystal forms in three differ...

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Autori principali: Gabrielsen, Mads, Zetterström, Caroline E., Wang, Dai, Beckham, Katherine S. H., Elofsson, Mikael, Isaacs, Neil W., Roe, Andrew J.
Natura: Artigo
Lingua:Inglês
Pubblicazione: International Union of Crystallography 2010
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2998365/
https://ncbi.nlm.nih.gov/pubmed/21139206
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309110039679
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