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Transfection of mammalian cells with plasmid DNA by scrape loading and sonication loading.

Scrape loading and sonication loading are two recently described methods of introducing macromolecules into living cells. We have tested the efficacy of these methods for transfection of mammalian cells with exogenous DNA, using selection systems based either on resistance to the drug G418 (Genetici...

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Bibliographic Details
Published in:Proc Natl Acad Sci U S A
Main Authors: Fechheimer, M, Boylan, J F, Parker, S, Sisken, J E, Patel, G L, Zimmer, S G
Format: Artigo
Language:Inglês
Published: National Academy of Sciences 1987
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Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC299564/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2446324/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.84.23.8463
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