Reliability of Quantitative Real-Time PCR for Bacterial Detection in Cystic Fibrosis Airway Specimens

The cystic fibrosis (CF) airway microbiome is complex; polymicrobial infections are common, and the presence of fastidious bacteria including anaerobes make culture-based diagnosis challenging. Quantitative real-time PCR (qPCR) offers a culture-independent method for bacterial quantification that ma...

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Bibliographic Details
Main Authors: Zemanick, Edith T., Wagner, Brandie D., Sagel, Scott D., Stevens, Mark J., Accurso, Frank J., Harris, J. Kirk
Format: Artigo
Language:Inglês
Published: Public Library of Science 2010
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2994853/
https://ncbi.nlm.nih.gov/pubmed/21152087
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0015101
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