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Codon Preference Optimization Increases Heterologous PEDF Expression

Pigment epithelium-derived factor (PEDF) is widely known for its neurotrophic and antiangiogenic functions. Efficacy studies of PEDF in animal models are limited because of poor heterologous protein yields. Here, we redesigned the human PEDF gene to preferentially match codon frequencies of E coli w...

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Bibliographic Details
Main Authors: Gvritishvili, Anzor G., Leung, Kar Wah, Tombran-Tink, Joyce
Format: Artigo
Language:Inglês
Published: Public Library of Science 2010
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2994832/
https://ncbi.nlm.nih.gov/pubmed/21152082
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0015056
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