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Codon Preference Optimization Increases Heterologous PEDF Expression
Pigment epithelium-derived factor (PEDF) is widely known for its neurotrophic and antiangiogenic functions. Efficacy studies of PEDF in animal models are limited because of poor heterologous protein yields. Here, we redesigned the human PEDF gene to preferentially match codon frequencies of E coli w...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2994832/ https://ncbi.nlm.nih.gov/pubmed/21152082 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0015056 |
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