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Efficient introduction of plasmid DNA into Trypanosoma brucei and transcription of a transfected chimeric gene.

Electroporation induces efficient transient transfection of Trypanosoma brucei, and the introduced DNA can be transcribed into RNA. When we delivered a high-voltage electric pulse to cells mixed with radiolabeled pBR322, approximately equal to 15% of the plasmid DNA was taken up by the parasites. Wh...

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Détails bibliographiques
Auteurs principaux: Eid, J, Sollner-Webb, B
Format: Artigo
Langue:Inglês
Publié: 1987
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC299400/
https://ncbi.nlm.nih.gov/pubmed/3479766
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