Site-directed mutagenesis of the nitrogenase MoFe protein of Azotobacter vinelandii

A strategy has been formulated for the site-directed mutagenesis of the Azotobacter vinelandii nifDK genes. These genes encode the α and β subunits of the MoFe protein of nitrogenase, respectively. Six mutant strains, which produce MoFe proteins altered in their α subunit by known single amino acid...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Brigle, Kevin E., Setterquist, Robert A., Dean, Dennis R., Cantwell, John S., Weiss, Mary C., Newton, William E.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1987
Assuntos:	Biological Sciences: Biochemistry
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC299230/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16593879/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.84.20.7066
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