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Overproduction and purification of the luxR gene product: Transcriptional activator of the Vibrio fischeri luminescence system

Expression of Vibrio fischeri luminescence genes requires an inducer, termed autoinducer, and a positive regulatory element, the luxR gene product. A plasmid containing luxR under control of a tac promoter was engineered to overproduce this gene product. The overproduced luxR gene product was active...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Principais autores: Kaplan, Heidi B., Greenberg, E. P.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1987
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC299138/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16578817/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.84.19.6639
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