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Use of a synthetic peptide antigen to generate antisera reactive with a proteolytic processing site in native human proinsulin: demonstration of cleavage within clathrin-coated (pro)secretory vesicles.

Polyclonal antibodies reactive with a cleavage site in human proinsulin (HPI) (C-peptide-A-chain junction) have been raised (rabbit, guinea pig) using a synthetic peptide antigen coupled with keyhole limpet hemocyanin. These antisera recognize native HPI and des-31,32-HPI equally well but react 20-5...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Steiner, D F, Michael, J, Houghten, R, Mathieu, M, Gardner, P R, Ravazzola, M, Orci, L
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1987
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC299034/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3306670/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.84.17.6184
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