Development of a Versatile Procedure Based on Natural Transformation for Marker-Free Targeted Genetic Modification in Streptococcus thermophilus

A versatile natural transformation protocol was established for and successfully applied to 18 of the 19 Streptococcus thermophilus strains tested. The efficiency of the protocol enables the use of in vitro-amplified mutagenesis fragments to perform deletion or insertion of large genetic fragments....

Πλήρης περιγραφή

Αποθηκεύτηκε σε:
Λεπτομέρειες βιβλιογραφικής εγγραφής
Κύριοι συγγραφείς: Fontaine, Laetitia, Dandoy, Damien, Boutry, Céline, Delplace, Brigitte, de Frahan, Marie Henry, Fremaux, Christophe, Horvath, Philippe, Boyaval, Patrick, Hols, Pascal
Μορφή: Artigo
Γλώσσα:Inglês
Έκδοση: American Society for Microbiology (ASM) 2010
Θέματα:
Genetics and Molecular Biology
Διαθέσιμο Online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2988589/
https://ncbi.nlm.nih.gov/pubmed/20935129
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01671-10
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