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Recruitment of substrate-specificity properties from one enzyme into a related one by protein engineering.
The Bacillus licheniformis and Bacillus amyloliquefaciens subtilisins differ by 31% in protein sequence and by factors of greater than 60 in catalytic efficiency, kcat/Km, toward various substrates. Despite large differences in sequence and substrate specificity for these serine proteases, only two...
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Главные авторы: | , , , |
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Формат: | Artigo |
Язык: | Inglês |
Опубликовано: |
1987
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Предметы: | |
Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC298815/ https://ncbi.nlm.nih.gov/pubmed/3299378 |
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